An analytical method for screening and confirming the presence of 84 pharmaceuti- cals and 23 labeled standards in water samples for a total of 107 components was developed using the Agilent 6460 Triple Quadrupole Mass Spectrometer (Triple Quad MS) with Jet Stream Technology.
The document details a developed analytical method for screening and confirming the presence of 84 pharmaceuticals and 23 labeled standards in water samples. The method utilizes the Agilent 6460 Triple Quadrupole Mass Spectrometer with Jet Stream Technology, demonstrating a significant enhancement in limits of detection compared to previous models.
The note outlines specific liquid chromatography (LC) conditions for five different analyte groups, including column types, mobile phases, flow rates, and gradients. It also provides optimized mass spectrometry parameters such as fragmentor voltage, MRM transitions, and collision energies for the analysis of various compounds.
| Column (Group 1-3) | Agilent ZORBAX Eclipse Plus C-18 narrow bore, 2.1 mm x 100 mm, 1.8 pm |
|---|---|
| Column (Group 4) | Agilent ZORBAX Eclipse HILIC Plus narrow bore, 2.1 mm x 100 mm, 3.5 pm |
| Column Temperature | 25 °C |
| Injection Volume | 15 pl |
| Gas Heater Temperature | 250°C |
| Sheath Gas Flow | 10 L/min |
The method uses the Agilent 6460 Triple Quadrupole Mass Spectrometer with Jet Stream Technology.
The method analyzes a total of 107 components, including 84 pharmaceuticals and 23 labeled standards.
The analysis time was reduced from a total of 90 minutes to less than 30 minutes.