Agilent Multiple Affinity Removal Columns – for Mouse Serum Proteins Instructions, is an instruction manual for a product of Agilent company. This product can remove high-abundance proteins from serum, leaving the low-abundance proteins for downstream proteomics analysis.
This document provides instructions for the Agilent Multiple Affinity Removal System designed for mouse serum proteins. The system utilizes antibody-antigen interactions on affinity removal columns to selectively remove high-abundant proteins such as albumin, IgG, and transferrin from crude biological fluids like serum and plasma.
The manual details the components of the system, including two column sizes (4.6x50-mm and 4.6x100-mm) and a starter kit containing optimized buffers, spin filters, and protein concentrators. It outlines a full protocol for loading samples, collecting flow-through fractions enriched with low-abundance proteins, eluting bound proteins, and regenerating the columns using an LC system.
| Column Size 1 | 4.6x50-mm |
|---|---|
| Column Size 2 | 4.6x100-mm |
| Target Proteins Removed | Albumin, IgG, and Transferrin |
| Flow Rate (Equilibration) | 1 mL/min |
| Storage Temperature | 2-8 °C (35-46 °F) |
The system removes high-abundant proteins including albumin, IgG, and transferrin.
No, you must not freeze the column; it should be stored in a refrigerator at 2-8 °C.
The capacity is approximately 37-50 µL of mouse serum.