Agilent Brilliant QPCR Master Mix Instruction Manual

This document is the instruction manual for Agilent's Brilliant QPCR Master Mix. It is for research use only and not for use in diagnostic procedures. The product comes with a limited warranty that only covers replacement of the product. Agilent is not liable for any direct, indirect, consequential, or incidental damages arising from the use, results of use, or inability to use this product.


Agilent Brilliant QPCR Master Mix Instruction Manual - cover page
Brand
Agilent
Document type
User Manual
Language
English
Pages
22
File format
PDF
File size
256 KB
Published
06 June, 2012
Updated
29 September, 2023
MD5 checksum
7CC208245061C8B06FC996B62FBE880F

About this manual

The document provides instructions for the Agilent Brilliant QPCR Master Mix, a single-tube reagent designed for high-throughput quantitative PCR. The kit includes 2x master mix and a reference dye sufficient for up to 400 reactions, intended for use with spectrofluorometric thermal cyclers.

What's inside

  • Materials Provided
  • Storage Conditions
  • Additional Materials Required
  • Molecular Beacons
  • TaqMan Probes
  • Endpoint vs. Real-Time Measurement
  • Preprotocol Considerations
  • PCR Cycling Programs
  • Troubleshooting

Specifications

Reactions per single kit400, 25-µl reactions
Storage temperature (components)-20°C
Storage temperature (after thawing)4°C

Frequently asked questions

What additional equipment is required?

A spectrofluorometric thermal cycler and nuclease-free PCR-grade water are required.

Is the reference dye light sensitive?

Yes, the reference dye is light sensitive and should be kept away from light whenever possible.

How long is full activity guaranteed after thawing?

Full activity is guaranteed for 6 months once the master mix has been thawed.

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