This document is the instruction manual for Agilent Brilliant QPCR Core Reagent Kit, which introduces the use of the product, precautions, etc.
This document is an instruction manual for the Agilent Technologies Brilliant QPCR Core Reagent Kit (Catalog #600530). The kit contains SureStart Tag DNA polymerase, core PCR buffer, magnesium chloride, dNTP mix, and a reference dye. It is designed for quantitative PCR applications using fluorescent probe technologies such as Molecular Beacons and TaqMan probes to detect genomic DNA, plasmid DNA, and cDNA targets.
| SureStart Taq DNA polymerase concentration | 5 U/ul |
|---|---|
| Core PCR buffer concentration | 10x |
| Magnesium chloride concentration | 50 mM |
| dNTP mix concentration | 20 mM (5 mM each of dATP, dITP, dGTP, and dCTP) |
| Reference dye concentration | 1 mM |
A thermal cycler and fluorescence reader or a spectrofluorometric thermal cycler are required.
Yes, a heated lid is strongly recommended because it allows optimal detection of the fluorescence.
Nuclease-free PCR-grade water is required.