pSG5 Vector instruction manual.
The document serves as an instruction manual for the Agilent pSG5 Vector (Catalog #216201). It outlines the materials provided with the product, specific storage requirements for the vector and bacterial stock, and details on preparing the host strain AG-1. The guide also includes recipes for LB broth and agar media necessary for culturing the bacteria.
The pSG5 Vector is a eukaryotic expression vector constructed by combining pKCR2 and the Agilent pBS vector.
The AG1 bacterial glycerol stock should be stored at –80°C.
The preparation may be stored at –20°C for 1–2 years or at –80°C for more than 2 years.