Introduces the characteristics and usage methods of ArcticExpress RIL Competent Cells and ArcticExpress RP Competent Cells
This document provides instructions for the use of Agilent ArcticExpress RIL and ArcticExpress RP competent cells. It details the materials provided in the kit, including specific catalog numbers for different cell types (DE3RIL, DE3RP, RIL, RP) and their guaranteed transformation efficiencies.
The manual outlines storage conditions requiring -80°C preservation and lists additional materials required for experiments, such as low-temperature incubators and specific reagents. It includes step-by-step protocols for overcoming protein misfolding, codon bias issues, and general protein expression methods.
| Efficiency (ArcticExpress DE3RIL/RP) | 25 x 10^8 cfu/ug pUC18 DNA |
|---|---|
| Efficiency (ArcticExpress RIL/RP) | 25 x 10^8 cfu/ug pUC18 DNA |
| Storage Temperature (Cells) | -80°C |
Cells must be stored immediately at -80°C and should not be placed in liquid nitrogen.
A low-temperature (10—13°C) incubator with a shaker and 14-ml BD Falcon polypropylene round-bottom tubes are required.