This document describes a protocol for the purification of IgG antibodies using the Bravo Automated Liquid-Handling Platform and Bravo 96AM Head with 96 PA-W AssayMAP® Bravo cartridges from Agilent Technologies. The document provides detailed information on the features and usage of PA-W AssayMAP Bravo cartridges.
This document provides a step-by-step protocol for performing protein A purification of IgG antibodies on the Agilent Bravo Automated Liquid Handling Platform. The procedure utilizes the Bravo 96AM Head and 96 PA-W AssayMAP Bravo cartridges, which contain a proprietary resin bed protected by a coating that requires rehydration before use.
The guide covers the entire workflow, including preparing equipment and materials (such as PBS buffer, elution buffer, and specific microplates), initializing the platform via VWorks software, setting up the cartridge receiver plate, configuring the protein A protocol parameters, arranging the Bravo deck layout, and performing post-purification analysis.
The Bravo Platform must have a computer running the VWorks software v11.1 or later.
Each AssayMAP Bravo protein A cartridge contains a 5-µL bed packed with protein A resin.