Industrial

Comparison of several LC/MS techniques for use in toxicology

This application note describes amino acid analysis methods using Agilent ZORBAX Eclipse Plus C18 columns for a variety of Agilent LC instrumentation and separation goals. The methods utilize a proven ortho-phthalaldehyde/9-fluorenyl- methyl chloroformate (OPA/FMOC) derivatization and take advantage of the Agilent 1200 Series SL and Agilent ZORBAX Eclipse Plus C18 stationary phase columns.

This article introduces a method for studying DNA sequence copy number alterations, which uses a new labeling technology to extract DNA from formalin-fixed, paraffin-embedded (FFPE) tissues and obtain high-quality data.

This Application Note describes the separation of nine food additives, acesulfame K, aspartame, caffeine, benzoic acid, sorbic acid, saccharine, trans-cinnamic acid and vanillin using the Agilent 1290 Infinity LC system. The ability of the Agilent 1290 Infinity LC pump to deliver mobile phases at high pressures of up to 1200 bars enables separation of the nine selected additives within a run time of 1 min. The Agilent 1290 Infinity Diode Array Detector (DAD) equipped with Agilent’s proprietary Max-Light flow cell provides sufficient sensitivity for reliable analysis of even 1 µL of the sample. The developed method was applied for the screening of additives present in soft drinks and colas.

Agilent Poroshell 120 columns offer the highest efficiency, while significantly reducing back pressure

This article describes a method for screening for EU banned dyes in textiles using the Agilent 1120 Compact LC with an Agilent 6140 Single Quadrupole LC/MS system and the Analytical Studio Browser software. The method allows for quick identification of samples that fail regulatory requirements, increasing productivity.

Analysis of phenolic antioxidants and erucamide slip additives in polypropylene formulations using Agilent 1120 Compact LC and Agilent Method Translator to obtain ASTM- equivalent methods

This note describes how to generate compound retention times that are permanent and universal through Retention Time Locking using the Agilent 7890A GCs, Agilent 5975 series MSD and Agilent 7000 series triple quadrupole MS systems

This study used an Agilent HPLC-Chip coupled with an Agilent Accurate-Mass Q-TOF LC/MS to analyze the glycopeptide and glycan moiety of a monoclonal antibody (mAb). The tryptic peptide map of the mAb was generated and the glycopeptide was assigned using accurate mass measurement. The enzymatically released glycan moiety from the mAb was extracted and analyzed on an HPLC-Chip coupled with a Q-TOF. Diagnostic oxonium ions from a glycan moiety were further used to identify the glycopeptide and the glycan. The superior mass accuracy of the LC/MS platform, combined with the powerful data processing capabilities of Agilent MassHunter and BioConfirm software, enabled identification of the glycopeptides and glycan moiety attached to the protein complex.

This document is an application compendium on analyzing and identifying impurities in drug substances using Agilent instrumentation. It provides guidance on improving overall workflow with Agilent products and applications. The compendium includes application notes on analyzing organic impurities with various Agilent LC and GC systems, analyzing residual solvents, analyzing inorganic impurities with ICP-MS, and determining impurities with LC/MS. Agilent's reliable instrumentation and services, including instrument qualification and diagnostic programs, ensure accurate and dependable impurity analysis. The analysis of impurities in drug substances is becoming increasingly challenging, from initial screening to routine quality control and assurance. Impurities are addressed from chemical aspects, including classification and identification.

This Application Note describes a method for the long-term detection of anabolic steroid metabolites in urine using both the Agilent 6890N network GC system with an Agilent 5975B Series GC/MSD and the Agilent 7000 Series Triple Quadrupole GC/MS. Monitoring the sulfate conjugates of these metabolites, instead of the routine glucuronide metabolites, allowed detection of methenolone for a post-administration period that was almost twice as long. This was achieved using GC/MS in SIM mode. Performing the analysis on the Triple Quadrupole GC/MS in SRM mode extends the detection period even further, to almost three times as long as the conventional glucuronide approach.

This Application Note describes the development of a fast method for the determination of 13 aldehyde and ketone derivates with the Agilent 1290 Infinity LC system. The method, which used acetone as organic co-solvent separates the analytes within 3.5 minutes.

This Application Note investigates the ability of a novel polymeric mixed mode strong anion exchange resin to extract acidic, neutral and basic drugs from urine

This document discusses the combination of Agilent ICP-MS ChemStation and Agilent OpenLAB ECM to comply with 21 CFR Part 11. The User Access Control Pack enables system access control and audit trail functionality, while Agilent OpenLAB ECM ensures secure record keeping and data archival. This solution has been successfully deployed at many leading life sciences companies to meet compliance requirements for 21 CFR Part 11.