Agilent Manuals

This document introduces a high-efficiency method for separating nucleosides using reversed-phase separation with protected-bonded-phase columns.

This document describes the method for catecholamine analysis using Agilent ZORBAX SB-C18 column

This document describes the analysis method for ergosterol, a pro-vitamin of Vitamin D2, in soil extracts. Ergocalciferol (Vitamin D2) is produced by the ultraviolet irradiation of ergosterol in plants, fungi, and yeasts. The use of Agilent ZORBAX Rapid-Resolution Rx-C18 columns enables fast analysis of soil extracts with good peak shape and retention.

This Application Note describes how an evaporative light scattering detector (ELSD) can be used with the Agilent 1100 Series purification system for peak-based fraction collection. The system setup is described, and an example is shown of how an ELSD can be used with the Agilent 1100 Series purification system for peak-based fraction collection.

This application note describes how to isolate active natural products from red clover using the Agilent 1100 Series purification systems.

This Application Note illustrates how to isolate and purify reaction byproducts using the Agilent 1100 Series purification system (AS) and demonstrates that large-scale pooling can be elegantly facilitated by incorporating the funnel tray into the Agilent 1100 Series fraction collector AS.

This document introduces a method for detecting the purity of synthetic �-homo polylysine (250) using capillary electrophoresis. Experimental results show that the purity of synthetic �-homo polylysine (250) in 50 mM phosphate buffer (pH 2.0) solution is greater than 99.7%.

Peak 2 was correctly identified to contain only tyrosine, peak 3 contained neither tyrosine nor tryptophan and peaks 4 and 6 contained only tryptophan (figure 1). Second derivative spectra can be used to emphasize small spectral differences in similar molecules. This is especially useful for identifying tryptophan and tyrosine residues in peptides (figure 2).

This application note briefly introduces derivative spectroscopy and illustrates its features and applications using computer-generated examples.

This application brief describes the application of CEC to the quantitative analysis of parabenes in body lotion. The method needed minimal sample preparation and provided quantitative data.

This application note presents an alternative method for dissolution testing using ultraviolet-visible spectroscopy instead of liquid chromatography.

This Application Note describes the peak-based purification of 15 samples, which model a part of a combinatorial chemistry library, using the Agilent 1100 Series purification system. The samples were purified using a generic method with a gradient from 5 to 95 % organic phase in relatively short time. The re-analysis of the fractions was done on an analytical HPLC system to check the collected fractions for purity of the desired compounds.

This Application Note describes purification of a recombinant GFP fusion protein and purity verification using fluorescent measurement, SDS-PAGE followed by Western blotting, and the Protein 200 assay.