Agilent Manuals

This document describes the solid phase extraction method for anabolic steroids using the AccuBOND II ODS (C18) 6 mL/500 mg cartridge. The sample is processed by adding methanol and water, followed by adsorption, elution, and concentration using vacuum. The residue is dissolved and injected into a gas chromatograph for analysis.

This application brief describes a solid phase extraction method for the analysis of benzodiazepines in serum using an Agilent AccuBOND SPE ODS (C18) 6 mL/500 mg cartridge.

This document describes the analysis of amino acids in seasonings using the automated pre-column derivatization method. Compared to post-column derivatization or manual pre-column derivatization methods, this method offers advantages such as simple system configuration, low running costs, and good stability of quantitative data.

The Agilent 2100 bioanalyzer and the Cell Fluorescence LabChip® kit can be used to determine the efficiency of transfection of mammalian cells using green fluorescent protein (GFP) as a reporter molecule.

This application note describes in detail how the Agilent Technologies 2100 bioanalyzer can be used to analyze PCR fragments using the DNA 7500 LabChip® kit. Both size and concentration information can be obtained for fragments ranging from 100–7500 base pairs. Sizing and quantitation accuracy were determined and performance was compared to slab gel electrophoretic methods. The advantages of the DNA 7500 assay for determining PCR product purity are also described.

This application note describes how the DNA 12000 LabChip® kit can be used with the Agilent Technologies 2100 bioanalyzer to size DNA fragments generated by restriction enzyme digestion. Sizing accuracy was found to be greater than 92 % for all samples analyzed. Compari- son of the chip-based separations to those obtained using agarose gel electrophoresis indicates that the DNA 12000 assay accurately sizes fragments over a wider range than can be usually analyzed on a slab gel.

The sequencing of oligosaccharides in glycosylated natural products was successfully investigated using liquid chromatography-mass spectrometry with electrospray ion source in negative ion mode.

This Application Note describes results in protein impurity testing and simultaneous molecular mass characterization with the Agilent 1100 Series GPC-SEC analysis system.

The document describes the detection of the major metabolite of cocaine in the hair of 2000-year-old Chilean mummies. This is particularly important in relation to Rohypnol® because victims of the drug may not immediately realize they have been violated. However, hair analysis is challenging due to the difficulty in digesting the matrix and the low concentrations of the metabolite. Therefore, the sensitivity must be in the picogram per milligram range. The metabolism of flunitrazepam produces the 7-aminoflunitrazepam metabolite through the reduction of the nitro-group. Flunitrazepam is a benzodiazepine marketed by Hoffman-La Roche under the brand name of Rohypnol®. It is not legally used in the United States, but its abuse has been increasing in the southern and southwestern regions. The relatively low street price and high potency of Rohypnol® are of particular concern.

Absolute quantitation with the Agilent 2100 Bioanalyzer and the Protein 200 LabChip® kit. The Protein 200 LabChip® kit allows sizing and quantitation of proteins ranging in size from 14 to 200 kD. Relative quantitation is performed in comparison to the upper marker, used as internal standard in each sample. However, the accuracy of the relative quantitation is affected by the staining efficiency of the protein dye. Absolute quantitation is enabled with the Agilent 2100 bioanalyzer software (revision A.02.01) using the Protein 200 assay in combination with protein standards of known concentrations. Absolute quantitation improves quantitation accuracy by eliminating the differences in staining efficiency among proteins. This Application Note describes how to perform absolute quantitation with the Protein 200 assay and demonstrates the obtained improvement in accuracy.

This document describes a method for purifying human erythropoietin (EPO) from formulated drugs using reversed-phase chromatography. This method allows EPO to be purified to homogeneity for further analysis by peptide mapping, mass spectrometry and protein sequencing.

This document introduces a method for optimizing cRNA fragmentation using the Agilent 2100 bioanalyzer for microarray experiments.

This document introduces the Agilent 2100 bioanalyzer and LabChip DNA analysis technology, which has the advantages of high speed, high precision, and ease of use