Agilent Manuals

This document introduces how to quickly identify o-rings, seals and gaskets using the handheld Agilent 4100 ExoScan FTIR analyzer.

This document introduces the analysis methods of low molecular weight resins from Agilent Technologies, Inc., including molecular weight distribution, composition, crystallinity and aggregation.

Fast separations of intact and reduced monoclonal antibodies (mAbs) were achieved with the use of an Agilent ZORBAX Rapid Resolution High Definition (RRHD) 300 diphenyl reversed-phase column and optimized chromatographic conditions. The unique diphenyl phase and robust rapid resolution high definition column technology, in combination with optimized gradient conditions, provided ultra fast separations and delivered excellent peak shapes demonstrating utility for high throughput mAb charac terizations.

This application note demonstrates the use of Agilent's Mx3005P QPCR System and the resDNASEQ Quantitative CHO DNA Kit (Life Technologies) for the detection of residual CHO host cell DNA. This method produces more specific, more sensitive, and faster results than traditional blot hybridization methods.

This application note demonstrates the applicability of SFC for typical polymer additives using the Agilent 1260 Infinity Analytical SFC System in combination with an Agilent 6130 Single Quadrupole LC/MS System.

This Application Note demonstrates that stringent sensitivity requirements for the detection of potentially harmful primary aromatic amines can be fulfilled when using an Agilent 1290 Infinity LC System equipped with the 1290 large volume injection kit. This enables the Agilent 1290 Infinity Autosampler for injection volumes up to 120 μL for sample enrichment on the column. A high sensitivity 60-mm Max-Light flow cell in the Agilent 1290 Infinity Diode Array Detector gives additional detection sensitivity.

This document describes a comprehensive method for profiling free and conjugated estrogens using capillary electrophoresis-time of flight-mass spectrometry. The method is sensitive and robust.

This article introduces two methods for detecting toxins in wheat using different sample preparation methods, a modified QuEChERS method and the Agilent Bond Elut mycotoxin SPE method. The results showed that both methods can effectively detect toxins in wheat, but the modified QuEChERS method is faster and uses less solvent and sorbent.

A miniaturized automated sample preparation method is described for the determination of fatty acid methyl esters in blood or plasma samples using the Agilent 7693 Series Automatic Liquid Sampler. Fifty-microliter samples are derivatized using acetyl chloride in methanol and the fatty acid methyl esters are extracted in iso-octane. Extracts are analyzed by GC-FID. Excellent repeatability is obtained and the method can be applied for fully automated fatty acid profiling in blood and plasma samples during dietary, biomedical or metabolomic studies.

This application note describes a chemical derivatization procedure required to facilitate measurement of steroidal analogs in electron capture negative chemical ionization mass spectrometry and the GC/MS/MS conditions for highly sensitive and selective analysis

Ion exchange is a commonly used technique for the separation of complex protein mixtures. This application note demonstrates how analysis times can be significantly reduced, increasing throughput without compromising analytical performance, by exploiting the benefits of small particle size, non-porous ion exchange sorbents.

The document describes the use of the Agilent 6550 iFunnel Q-TOF LC/MS system for high-throughput, high-efficiency metabolome profiling. Compared to the previous generation Q-TOF instrument, this platform has increased sensitivity and resolution, resulting in the detection of an order of magnitude more ions. It provides a 6 to 7-fold increase in coverage of the E. coli metabolome and more detailed pathway analysis.

Agilent Bio SEC columns are used to separate proteins, oligonucleotides, and other complex biopolymers. This application note demonstrates the effect of pore size on separations of proteins and antibody using Agilent Bio SEC-3 HPLC columns.

The Application Note describes the analysis of paracetamol and aspirin to demonstrate that typical accep- tance criteria for a USP method can be fulfilled.