Agilent Manuals

This document describes the analysis of Nortriptyline in plasma. It utilizes on-line extraction and separation using a ZORBAX Eclipse XDB-C8 column, which provides excellent peak shape. The method operates optimally over a wide pH range. This fast and direct approach is used to determine physiological levels of Nortriptyline in the bloodstream.

This application note describes a HPLC method for the analysis of methotrexate. The method uses a 10/90 acetonitrile/pH 6.0 phosphate/citrate buffer mobile phase and a ZORBAX Eclipse XDB-C18 column, column dimensions 3.9 x 300 mm, particle size 10 µm.

The document describes the USP method for analyzing Buspirone in pharmaceutical applications using HPLC. It highlights the use of ZORBAX Eclipse XDB-C18 column for improved peak shape and higher efficiency.

This document describes how new technologies can achieve excellent peak shape in the separation of tricyclic antidepressants using the Agilent XDB-C8 column and a mobile phase containing THF.

This is an application note on the use of tetracycline on the Agilent ZORBAX Rx-C8 column, including peak shape and resolution.

The document describes the method and advantages of using ZORBAX Eclipse XDB-C18 for HPLC analysis. By using ZORBAX Eclipse XDB-C18, improved peak shape, higher separation efficiency, greater sensitivity, reduced analysis time, reduced back pressure, and reduced solvent use per analysis can be achieved. The performance of this product surpasses the requirements of USP, with a higher number of theoretical plates and a closer tailing factor to the ideal value.

This application notes updates the USP methods for metronidazole to improve column efficiency and sensitivity.

This document describes the rapid analysis of dexamethasone using the USP method. By choosing a ZORBAX Rapid-Resolution column configuration, analysis time is reduced by 70% and solvent consumption is subsequently reduced. Improved performance can be achieved by selecting an alternative column configuration.

This document describes the high-resolution separation method and characteristics of nonsteroidal anti-inflammatory drugs, as well as the narrow-bore column configuration suitable for LC/MS applications. The Eclipse XDB-C8 column is capable of separating acidic, basic, and neutral molecules with good peak shape. Specific analysis conditions and the pKa values of the drugs are provided.

This document describes the separation method for tricyclic antidepressants and their metabolites. It explains that good peak shapes can be obtained for very basic compounds at pH 6.2 by adding TEA. The document also suggests that changing the pore-size and surface area of the column packing can help achieve the desired separation. Specific analysis conditions and methods are provided.

The document describes the separation method for extracting morphine and its metabolites from blood plasma. Good resolution and peak shape for morphine and two metabolites can be achieved using ZORBAX SB-C18 at moderate pH. Morphine and the metabolite morphine-6-glucuronide can be sensitively detected using electrochemical detection, while morphine-3-glucuronide can be sensitively detected using fluorescence.

This document describes a method for high-resolution chiral separation of R- and S-Norfluoxetine using Ultron ES-Pepsin. The method achieves excellent separation and extends the column's useful lifetime.

This document describes the separation of cephalosporins on Agilent ZORBAX XDB-C8 column.