Agilent Manuals

This Application Note investigates the ability of a novel polymeric mixed mode strong anion exchange resin to extract acidic, neutral and basic drugs from urine

This document discusses the combination of Agilent ICP-MS ChemStation and Agilent OpenLAB ECM to comply with 21 CFR Part 11. The User Access Control Pack enables system access control and audit trail functionality, while Agilent OpenLAB ECM ensures secure record keeping and data archival. This solution has been successfully deployed at many leading life sciences companies to meet compliance requirements for 21 CFR Part 11.

This Application Note illustrates the possibilities of the Agilent 1290 Infinity LC system and the Agilent 6460A Triple Quadrupole Mass Spectrometer for quantitative analysis of poten- tial genotoxic impurities (PGIs) in active pharmaceutical ingredients (APIs). A generic approach was used to determine arylamine and aminopyridine PGIs in selected APIs. The influence of column length and mobile phase composition on the recovery of these impuri- ties in real samples were investigated. High resolution UHPLC using a 150 mm RRHD col- umn packed with 1.8 µm particles resulted in the best overall performance. In cases of good separation between API and the target solutes, analysis speed and sample throughput could be increased using a 50 mm RRHD column. Detection limits were below 20 ppb (ng/g, relative to the API) using MS/MS and below 100 ppb (ng/g, relative to the API) with DAD for 2,6-dichloroaniline, which could not be detected with ESI-MS at trace levels.

Agilent's high efficiency GC columns can be used for PAH analysis. This article introduces the performance of two Agilent J&W columns, DB-EUPAH and DB-5ms. Both columns can separate PAHs well.

This document discusses the important impact of RNA quality on miRNA expression analysis, as well as the application of the Agilent 2100 Bioanalyzer and Agilent Small RNA Assay in RNA integrity assessment and miRNA analysis.

This application note describes a method for determining the N-nitrosamine content in rubber articles using the Agilent 7000A triple quadrupole GC/MS system. The method employs an internal standard, N-nitroso-N-dipropylamine (NDPA), for the trace-level detection and confirmation of seven target N-amines, N-nitrosodimethylamine (NDMA), N-nitrosodiethylamine (NDEA), N-nitrosodibutylamine (NDBA), N-nitrosopiperidine (NPIP), N-nitrosopyrrolidine (NPYR), N-nitrosomorpholine (NMOR), and N-nitroso-N-ethyl-N-phenylamine (NEPhA). The resulting low method detection limit, good linearity, and sample recovery demonstrate the high selectivity and sensitivity of the method described in this application note for trace-level detection and confirmation of the targeted N-amines in complex sample matrices.

A method was developed using ultra high performance liquid chromatography coupled to triple quadrupole mass spectrometry (LC/MS/MS) to quantitatively determine alprazolam in dried blood spots (DBS).

This Application Note describes a fast quality control method for the analysis of notoginsenoside R1 and Ginsenosides Rg1, Re and Rb1 using the Agilent 1290 Infinity LC system and an Agilent ZORBAX RRHD 1.8 µm column. Compared to conventional methods, the rapid method is much faster, and maintains the same performance and quality of separation. In addition, solvent consumption is also dramatically reduced.

4-Plex iTRAQ Based Quantitative Proteomic Analysis Using an Agilent Accurate -Mass Q-TOF

Detection and identification of impurities in pharmaceutical drugs using Agilent MassHunter software

The Agilent 1290 Infinity Multi-method Solution for LC allows analysis of seven different food applications on one instrument without column or mobile phase changes. It features automated switching between columns, solvent selection, and the ability to configure different separation conditions.

Fast LC methods can help to increase sample throughput significantly without compromising data quality.

This document introduces the Agilent 1200 Series LC Method Development Solution and Agilent Easy Access software LC walk-up system, which can be used for reaction kinetics, column scouting, and impurity checks with up to eight columns

This Application Note describes a method of injecting large sample volumes using an Agilent 1100 Series purification system and injection pump.