Agilent Manuals

The Precision Optical Performance AlInGaP LED lamps provide superior light output for excellent readability in sunlight and are extremely reliable. These LED lamps are untinted, nondiffused, T-13/4 packages incorporating second generation optics producing well defined spatial radiation patterns at specific viewing cone angles. These lamps are made with an advanced optical grade epoxy, offering superior high temperature performance.

This Application Note compares two suppliers Beckman Coulter and Advanced Analytical for separation matrices for protein characterization by capillary gel electrophoresis (CGE) with UV detection on the Agilent 7100 CE system. Two dif- ferent sample sets were analyzed: fi rst, a protein size standard and BSA as a test protein for molecular weight determination; second, a reduced antibody standard for quantifi cation of light chain, non-glycosylated heavy chain and heavy chain. To get an estimate for the intermediate precision, these experiments were performed with two different gel lots and capillary batches each. Across these experiments, the gels from both suppliers showed a similar performance. Impurity detection experiments were done with a low molecular weight protein spiked into a non-reduced antibody standard sample. With the gels of both suppliers it was possible to detect this protein down to a level of 0.1 %.

This Application Note describes a method for the quantifi cation of two antibacterial drugs, olaquindox and carbadox in animal feedstuff using the Agilent 1260 Infinity Binary LC system.

This application note describes a rapid method for detecting and quantifying mycotoxigenic fungi using the Stratagene Mx3000P QPCR system.

This application note describes a method for performing copy number analysis of archived FFPE tumor samples using Agilent Oligo Array CGH. The method uses Kreatech’s Universal Linkage System (ULS™) technology, a non-enzymatic direct labeling methodology, that can be used with degraded FFPE tissue-derived DNA. The method also features a simple, single-step sample preparation procedure, which reduces the cost of sample preparation. In addition, the method employs a novel labeling strategy that significantly improves the labeling efficiency of samples. Finally, the method is validated using a variety of FFPE tissue samples, and the results demonstrate that the method is capable of producing high-quality copy number data.

The Agilent 2100 Bioanalyzer is an easy-to-use benchtop system with ready-to-run kits for a wide range of applications.

The Agilent 2100 Bioanalyzer is an integrated, inexpensive, compliant solution for the analysis of nucleic acids, proteins, or cells. It is equipped with IQ, OQ/PV, and 21CFR part 11 compliance, making it suitable for quality control and analysis of antibodies, protein pharmaceuticals, and other biomolecules such as RNA and DNA.

A new method for the targeted analysis of proteins is presented that combines the specificity of immunoprecipitation with the sensitivity of protein detection on microchips using the High Sensitivity Protein 250 assay for the Agilent 2100 Bioanalyzer. As an alternative to Western blotting, this method is valuable for researchers engaged, for example, in protein expression and purification in pharma, biotech or academic labs. Advantages of this new method in comparison to Western blotting are: higher specificity and sensitivity, less manual steps and direct availability of quantitative data, 3 hours versus 1 day analysis time, 10x less primary and no secondary antibody needed, environmentally friendly process.

This application note describes how the Agilent Technologies 2100 Bioanalyzer can be used to analyze protein extracts from transgenic seedlines. Accuracy and precision in the determination of protein size and concentration was sufficiently good to allow for the characterization of experimental seedlines based solely on expressed protein profiles.

This application note describes the use of the Agilent 2100 bioanalyzer to quickly verify siRNA integrity and determine optimal transfection conditions for gene silencing experiments.

This study explored the interference of Sybr®Green I on the performance of Agilent 2100 bioanalyzer. The results showed that Sybr®Green I had little effect on the performance of the analyzer.

This article describes a method for flow cytometric analysis of human primary cells using the Agilent 2100 bioanalyzer and on-chip staining. The transfection efficiency of primary cells can be determined using green fluorescent protein (GFP) as a reporter molecule. The advantages of using the compact 2100 bioanalyzer for monitoring transfection efficiency in primary cells include low cell consumption, high reproducibility of results, fast on-chip staining procedure, and ease of use.

This application note describes the 5’-Nuclease PCR Assays for Foodborne Pathogen Detection using the Agilent-Stratagene Mx3000P Q-PCR System. This method is rapid, highly sensitive and specific.

This article describes an optimized analysis method for sizing agents used in paper production, using gel permeation chromatography combined with an evaporative light scattering detector for qualitative and quantitative analysis. This method allows for monitoring and controlling the level of sizing agents in paper sizing fluids.

An efficient plasticizer additive analysis method