This manual provides instructions for using the Takara RR392A/S Probe qPCR Mix, with UNG for Real Time PCR detection.
The document describes the Takara Probe qPCR Mix, with UNG (Code No. RR392A/S), a reagent for Real Time PCR (qPCR) detection using the probe method. It details the kit's components, including the 2×Premix reagent containing Hot Start PCR enzyme, dNTPs, Mg2+, Tli RNase H, and UNG, as well as ROX Reference Dyes for specific instruments. The manual explains the principle of operation, which involves monitoring PCR amplification in real-time using fluorescent probes. This manual is written in Chinese.
| Reaction Volume per Kit | 50 μl reaction x 200 times |
|---|---|
| Premix Concentration | 2×Conc. |
No, the kit does not contain probes and primers; they must be prepared separately.
The total volume for one reaction is 25 μl.
The 25°C step should last for 10 minutes.