TaKaRa DRR013A LA PCR Kit Ver.2.1 User Manual

This manual provides instructions for using the TaKaRa DRR013A LA PCR Kit for long and accurate amplification of DNA fragments up to 48 kbp.


TaKaRa DRR013A LA PCR Kit Ver.2.1 User Manual - cover page
Brand
TAKARA
Category
Other
Document type
User Manual
Language
Chinese
Pages
16
File format
PDF
File size
530 KB
Published
21 January, 2012
Updated
22 October, 2026
MD5 checksum
E811C03D4B616E4148365AB34C3D68F9

About this manual

The TaKaRa DRR013A LA PCR Kit Ver.2.1 is designed for the long and accurate amplification of DNA, capable of handling fragments up to 48 kbp. The kit includes essential components such as TaKaRa LA Taq enzyme, dNTP Mixture, and specialized buffers like 10×LA PCRTM Buffer II and GC Buffers for amplifying GC-rich or complex DNA structures. This manual is written in Chinese.

What's inside

  • Product Description
  • Kit Contents
  • Storage Conditions
  • LA PCRTM Technology Principle
  • Usage Precautions
  • Genomic DNA Purification
  • Experimental Procedures
  • Agarose Gel Selection
  • Q&A
  • References

Specifications

Amplification CapacityUp to 48 kbp
Reaction Volume50 μl
Enzyme Activity5 U / μl

Frequently asked questions

What is the maximum length of DNA that can be amplified?

The kit can amplify DNA fragments up to 48 kbp.

What is the recommended reaction volume for long fragment amplification?

The recommended reaction volume is 10 to 50 μl.

How should the kit components be mixed?

Components should be mixed gently by pipetting or inverting the microtube to avoid foaming or enzyme inactivation.

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