User manual for TaKaRa D103A pMD18-T Simple Vector, a TA cloning kit for efficient cloning of PCR products.
The TaKaRa D103A pMD18-T Simple Vector is a specialized vector designed for the efficient TA cloning of PCR products. Based on the pUC18 vector, it eliminates multiple cloning sites and incorporates an EcoR V site to facilitate subcloning. The kit includes the vector, a Control Insert, and a high-efficiency ligation solution (Solution I) that allows for direct transformation of bacteria after a short 30-minute reaction at 16°C. This manual is written in Chinese.
| Vector Size | 2,692 bp |
|---|---|
| Vector Concentration | 50 ng/ μl |
| Control Insert Volume | 10 μl |
| Solution I Volume | 75 μl |
| Control Insert Purity | >90% white colonies |
| Recommended Competent Cell Efficiency | ≥ 1×10^8 cfu/μg pUC19 |
Yes, but the reaction efficiency is slightly lower than at 16°C.
This may occur if the inserted DNA fragment is short (less than 500 bp) and does not affect the LacZ gene reading frame.
Universal primers used for pUC18 vector sequencing, such as BcaBEST Sequencing Primers or M13 Primers, can be used.