This manual describes the Takara D2680A PrimeScript Reverse Transcriptase, an RNase H- enzyme for synthesizing 1st-strand cDNA from RNA templates.
The document provides a product description for the PrimeScript Reverse Transcriptase (Code: D2680A), developed by TaKaRa. It is described as a novel RNase H-type reverse transcriptase with strong extension capabilities, suitable for synthesizing long-chain cDNA and constructing full-length cDNA libraries without requiring high-temperature reactions that might degrade RNA.
The manual details the product's packaging (10,000 Units), storage conditions (-20°C), and composition of the enzyme storage solution. It defines the activity unit based on [3H] dTTP incorporation and lists purity tests against DNA and RNA templates. Furthermore, it outlines experimental procedures for 1st-strand cDNA synthesis and provides specific examples using Mouse Heart Total RNA, HL60 Total RNA, and Mouse Liver Total RNA for RT-PCR and Real Time RT-PCR applications. This manual is written in Chinese.
The solution contains Tris-HCl (pH 7.8), NaCl, EDTA, DTT, and Glycerol.
The enzyme requires a reaction time of 30 to 60 minutes at 42°C.
The maximum usage of total RNA in a 10 μl reaction system is 500 ng.