One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) is a one-step, real-time reverse transcription PCR (RT-PCR) kit using probe detection. With this kit, all RT-PCR steps can be performed in a single tube. Therefore, it is not necessary to add additional reagents during the reaction, minimizing the risk of contamination and simplifying the workflow. Amplified products are monitored in real time without the need for gel electrophoresis after PCR. This kit is suitable for the detection of small amounts of RNA. This kit uses PrimeScript Reverse Transcriptase, which allows efficient and rapid cDNA synthesis, and TaKaRa Ex Taq® HS, which enables highly efficient hot start PCR. Compatible instrument systems include: Applied Biosystems 7300/7500 Real-Time PCR System (Thermo Fisher Scientific), LightCycler (Roche Diagnostics), Smart Cycler System/Smart Cycler II System (Cepheid) and Thermal Cycler Dice™ Real Time System II.
The One Step PrimeScript RT-PCR Kit (Perfect Real Time) is designed for one-step, real-time reverse transcription PCR (RT-PCR) using probe detection. This kit allows all RT-PCR steps to be performed in a single tube, minimizing the risk of contamination and simplifying the workflow. It is suitable for the detection of small amounts of RNA, such as viral RNA.
| Reaction System Volume | 50 μl |
|---|---|
| Reverse Transcriptase | PrimeScript Reverse Transcriptase |
| DNA Polymerase | TaKaRa Ex Taq HS |
| Compatible Instruments | Applied Biosystems 7300/7500, LightCycler, Smart Cycler, Thermal Cycler Dice |
The kit should be stored at -20℃.
No, this kit requires gene-specific primers for reverse transcription.
No, amplified products are monitored in real time without the need for gel electrophoresis.