The TaKaRa Code: DCY209 CycleavePCR® Legionella Detection Kit (25 μl reaction × 50 times) is a test kit that can quickly and specifically detect the mip (macrophage infectivity potentiator protein) gene of L.pneumophila and the 5S rRNA gene shared by Legionella species using the Cycling Probe method. It has the characteristics of high sensitivity, strong specificity, simplicity, and speed.
This manual provides detailed procedures for the CycleavePCR Legionella Detection Kit (code DCY209), including kit components, preparation of reaction mixes, and real‑time PCR settings. It also describes the interpretation of results using the mip gene, SS rRNA gene, and internal control signals.
Safety and handling notes are included, such as storage at –20 °C, contamination prevention measures, and the use of positive and negative controls to ensure reliable detection of Legionella spp. in environmental samples. This manual is written in Japanese.