Key facts
- Manuals
- 8
- Models
- 3
- Categories
- 1
- Languages
- ZH · JA
- Last added
- 2026-09-28
TAKARA models by category
Featured TAKARA manuals
Takara TB Green Premix Ex Taq II (Tli RNaseH Plus) User Manual
This manual describes the TB Green Premix Ex Taq II (Tli RNaseH Plus) reagent kit, a 2× premix for real‑time PCR that includes TB Green fluorophore and Tli RNaseH to improve specificity and quantitation. The kit provides 50 µl reactions for up to 200 runs and is compatible with a range of authorized real‑time PCR instruments.
The document includes product description, principle of the kit, contents, storage conditions, required reagents and instruments, safety precautions, detailed operation steps, and guidance for selecting experimental conditions. It also lists related Takara products and regulatory information. This manual is written in Chinese.
Covers: Product Description · Reagent Kit Principle · Contents · Storage · Required Reagents and Instruments · Precautions
TaKaRa LA Taq with GC Buffer User Manual
The LA Taq with GC Buffer kit from TaKaRa contains a high‑fidelity DNA polymerase with 3'‑5' exonuclease activity and two GC‑enhancing buffers. It is designed for efficient amplification of long or GC‑rich DNA fragments, delivering strong fidelity and the ability to clone PCR products directly into T‑vectors.
The manual details product contents, activity definition, purity criteria, recommended PCR setups, cycling parameters, and troubleshooting notes. It also includes guidelines for buffer selection, gel‑loading preparation, and example electrophoresis results. This manual is written in Chinese.
Covers: Product Contents · Activity Definition · Purity · Applications · PCR Performance · Application Notes and Precautions
TaKaRa DCY209 CycleavePCR Legionella Detection Kit User Manual
This manual provides detailed procedures for the CycleavePCR Legionella Detection Kit (code DCY209), including kit components, preparation of reaction mixes, and real‑time PCR settings. It also describes the interpretation of results using the mip gene, SS rRNA gene, and internal control signals.
Safety and handling notes are included, such as storage at –20 °C, contamination prevention measures, and the use of positive and negative controls to ensure reliable detection of Legionella spp. in environmental samples. This manual is written in Japanese.
Covers: Kit Contents · Preparation of Reaction Mixes · Real‑time PCR Setup · PCR Cycling Conditions · Positive and Negative Controls · Result Interpretation
TaKaRa LA Taq with GC Buffer Application Note
TaKaRa LA Taq is a high‑fidelity DNA polymerase with 3′→5′ exonuclease (proofreading) activity. The kit includes the enzyme and two GC‑rich buffers designed to improve amplification of difficult templates.
The note provides product contents, recommended PCR conditions, example protocols for human genomic DNA, and practical precautions such as storage at –20 °C and buffer selection for GC‑rich targets. This manual is written in Japanese.
Covers: Product Contents · PCR Performance · PCR Reaction Conditions · Example Applications · Precautions · Storage
Latest TAKARA manuals
Takara TB Green Premix Ex Taq II (Tli RNaseH Plus) User Manual
User Manual 14 pages · 698 KB · ZHInstruction manual for Takara TB Green Premix Ex Taq II (Tli RNaseH Plus) reagent kit, covering usage, storage, and instrument compatibility.
TaKaRa LA Taq with GC Buffer User Manual
User Manual 2 pages · 256 KB · ZHThe TaKaRa LA Taq with GC Buffer User Manual provides usage instructions, reaction conditions, and buffer preparation for high‑fidelity PCR.
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TaKaRa DCY209 CycleavePCR Legionella Detection Kit User Manual
User Manual 12 pages · 486 KB · JAInstructions for using the TaKaRa DCY209 CycleavePCR kit to detect Legionella DNA by real‑time PCR.
TaKaRa LA Taq with GC Buffer Application Note
Application Note 2 pages · 236 KB · JAThis application note explains how to use TaKaRa LA Taq with GC Buffer for high‑fidelity PCR of GC‑rich templates.
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Application Note
How to use the product for one specific task, with worked examples.
Questions answered in TAKARA manuals
At what temperature should the kit be stored for long‑term preservation?
Store the kit at –20 °C for long‑term preservation.
From: Takara TB Green Premix Ex Taq II (Tli RNaseH Plus) User Manual
How many reactions does the kit provide?
The kit provides 200 reactions of 50 µl each.
From: Takara TB Green Premix Ex Taq II (Tli RNaseH Plus) User Manual
What is the activity definition of one unit of LA Taq?
One activity unit (U) is defined as the amount of enzyme that leaves the DNA electrophoresis band unchanged under the test conditions.
Which buffer should be used first for PCR with GC‑rich templates?
Use Buffer I first; if amplification fails, try Buffer II.
What is the maximum amplicon size that can be amplified with this kit?
Up to 35 kbp can be amplified using 2XGC Buffer I.