User manual for the TaKaRa D322 kit, a Real Time PCR assay for detecting rabbit genomic DNA in food, cosmetics, and feed samples.
The TaKaRa Code D322 Real Time PCR Rabbit DNA Detection Kit is designed for the rapid identification of rabbit genomic DNA using dual-labeled fluorescence PCR technology. It targets the Cytochrome C oxidase subunit I (COX I) gene to detect rabbit-derived components in various samples such as food, cosmetics, and feed. The kit utilizes a two-channel synchronous detection system with FAM and HEX fluorescent dyes to simultaneously amplify the target gene and an internal control, ensuring reaction reliability and preventing false negatives. This manual is written in Chinese.
| Reaction Volume | 25 μl |
|---|---|
| Target Gene | Cytochrome C oxidase subunit I (COX I) |
| Control DNA Fragment Size | 111 bp |
| Internal Control DNA Fragment Size | 92 bp |
Compatible instruments include ABI PRISM 7000/7700/7900HT, 7300/7500 Real-Time PCR System, 7500 Fast Real-Time PCR System, LightCycler, Smart Cycler System, and Mx3000P.
The recommended kit for preparing genomic DNA is the TaKaRa Universal Genomic DNA Extraction Kit Ver. 3.0 (Code: DV811A).
A sample is considered to contain rabbit-derived components if FAM fluorescence is detected and the Ct value is ≤35, provided the Negative Control is normal.