Takara D6130 kit manual explains components, storage, principle, preparation, operation steps and example applications for cDNA synthesis.
The manual describes the Takara D6130 M‑MLV RTase cDNA Synthesis Kit, its components, storage conditions and the underlying Gubler‑Hoffman principle. It provides detailed pre‑reaction preparation, step‑by‑step protocols for first‑ and second‑strand synthesis, and methods for cDNA purification.
Example procedures include cloning of cDNA, ligation to linkers or adaptors, and insertion into λ‑vector. Precautions, reagent handling tips, and troubleshooting notes are also included. This manual is written in Chinese.
| Reverse Transcriptase (M‑MLV) activity | 200 U/μl |
|---|---|
| RNase Inhibitor activity | 20 U/μl |
| Oligo(dT)18 Primer concentration | 1 μg/μl |
| Random Primer (9mer) concentration | 0.3 μg/μl |
| dNTP mixture concentration | 10 mM each |
2 μg of poly(A)+ RNA is used as the template.