This document provides an instruction manual for TaKaRa LA Taq® with GC Buffer. The product is a heat-resistant DNA polymerase with 3′→5′ Exonuclease activity and Proof reading activity. It is suitable for PCR amplification of DNA. The product exhibits higher efficiency and fidelity in amplifying DNA fragments larger than 10 kbp, especially for templates with complex secondary structures and repetitive sequences. The use of GC Buffer can enhance the amplification. The product includes TaKaRa LA Taq, 2×GC Buffer I, 2×GC Buffer II, and 6×Loading Buffer.
The LA Taq with GC Buffer kit from TaKaRa contains a high‑fidelity DNA polymerase with 3'‑5' exonuclease activity and two GC‑enhancing buffers. It is designed for efficient amplification of long or GC‑rich DNA fragments, delivering strong fidelity and the ability to clone PCR products directly into T‑vectors.
The manual details product contents, activity definition, purity criteria, recommended PCR setups, cycling parameters, and troubleshooting notes. It also includes guidelines for buffer selection, gel‑loading preparation, and example electrophoresis results. This manual is written in Chinese.
| Packaging amount | 125 U |
|---|---|
| GC Buffer II concentration | 5 mM Mg2+ (2X) |
| Maximum amplifiable fragment (using Buffer I) | 35 kbp |
One activity unit (U) is defined as the amount of enzyme that leaves the DNA electrophoresis band unchanged under the test conditions.
Use Buffer I first; if amplification fails, try Buffer II.
The enzyme can efficiently amplify DNA fragments up to 35 kbp in length.