Agilent MassHunter BioConfirm software version B.03.01 combines high-performance LC/MS instrumentation with highly optimized software tools for rapid and accurate characterization of proteins and peptides. The software provides the industry's most accurate protein molecular weight determination, unrivaled speed for complex protein mixtures, and a unique feature-finding and deconvolution algorithm for the analysis of smaller molecules.
This document provides a technical overview of the Agilent MassHunter BioConfirm software version B.03.01, designed to solve challenges in recombinant antibody characterization and synthetic peptide confirmation when used with high-performance LC/MS instrumentation.
The software offers optimized tools for intact proteins and synthetic peptides, including maximum entropy deconvolution for accurate molecular weights and the Large Molecular Feature Extraction (LMFE) algorithm for rapid processing of complex mixtures. It also includes specific algorithms like MFE for smaller molecules such as peptide digests.
The LMFE algorithm takes 15 minutes to process an analysis that contains hundreds of proteins.
This algorithm delivers accurate molecular weights usually within 25 ppm.