The document introduces a material for assessing the purity of polyclonal antibodies after separation by affinity chromatography.
This document provides a technical overview of the PL-SAX 4000A material, which features an open pore structure optimized for separating very large molecules like immunoglobulins at high flow rates. It details a specific application where the material was used to assess the purity of polyclonal antibodies isolated from human serum using an affinity media, demonstrating the separation of bound and unbound fractions.
| Column Material | PL-SAX 4000A |
|---|---|
| Matrix Composition | polystyrene/divinylbenzene (PS/DVB) polymer |
| Coating | quaternized polyethyleneimine |
| Detection Wavelength | 280 nm |
| Flow Rate (Affinity) | 1.0 mL/min |
| Flow Rate (Anion Exchange) | 4.0 mL/min |
It is used for the optimum separation of very large molecules such as immunoglobulins.
The bound antibodies were eluted using 0.1 M glycine, pH 2.5, and the pH was adjusted to neutrality using sodium hydroxide.
No, this product is not cleared or approved by the United States FDA or other international regulatory agency for use in clinical diagnostic procedures.