This document mainly introduces the effect of molecular weight distribution on sample loading on the Agilent PLgel 10 µm MIXED-B column when separating polymer samples.
This document provides a technical overview of how sample loading in gel permeation chromatography (GPC) and size exclusion chromatography (SEC) depends on the molecular weight distribution of the polymer. It demonstrates that broader distribution polymers can be loaded to a greater extent.
| Column | Agilent PLgel 10 pm MIXED-B, 25 x 300 mm |
|---|---|
| Part Number | PL1210-6100 |
| Eluent | THF |
| Flow Rate | 10 mL/min |
| Detector | RI |
| System | Agilent PL-GPC 50 |
The flow rate used for the column calibration was 10 mL/min.
A solution of 200 pL was injected for the calibrants test.
The column dimensions are 25 x 300 mm.