This Technical Overview describes the online coupling of an Agilent 1260 Infinity LC and an Agilent 7890A GC via an Agilent 1200 Infinity Series 2-position/6-port switching valve using the solvent vent mode of the multimode inlet of the GC for solvent evaporation. The GC chromatogram of a cholesteryl fatty acid ester mixture obtained by the online LC-GC combination was comparable to that obtained using the Agilent 7890A GC as a single GC with split injection.
This document describes the technical implementation of on-line coupled liquid chromatography-gas chromatography (LC-GC). It details the setup using an Agilent 1260 Infinity LC connected to an Agilent 7890A GC via a multimode inlet (MMI) in solvent vent mode. The system utilizes an Agilent 1200 Series switching valve for fraction transfer.
The LC conditions specify an injection volume of 0.200 mL/min.
The GC oven temperature program starts at an initial temperature of 40 °C for 2 min.