Describes the advantages of Agilent Evaporative Light Scattering Detector (ELSD) over UV detector in analyzing succinic acid, including stable baselines, no drift and solvent peaks, and much greater response.
This document provides a technical overview of using the Agilent Evaporative Light Scattering Detector (ELS) for the analysis of succinic acid. It explains that succinic acid lacks a good UV chromophore, making UV detection difficult without denaturing the solute or requiring gradient elution which complicates separation from other substances like proteins.
The Agilent ELS detector is presented as a superior alternative, offering clean baselines, no drift, and no solvent peaks. The document details specific experimental conditions used to demonstrate these capabilities, including sample concentration, column specifications, eluent composition, and flow rate, showing that the ELS can detect succinic acid down to an on-column load of 2 yg.
| Sample Concentration | 1 mg/mL |
|---|---|
| Column Specification | PLRP- 300A 8 ym, 4.6 x 150 mm |
| Eluent A Composition | 1% ACN, 0.1% TFA, 99% Water |
| Eluent B Composition | 100% ACN, 0.1% TFA |
| Gradient Profile | 5-80% B in 20 min |
| Flow Rate | 1.0 mL/min |
| UV Detection Wavelength | 215 nm |
| Minimum Detection Limit | 2 yg on-column load |
Succinic acid does not have a good UV chromophore, and separating it from substances requiring gradient elution rules out RI detection.
The Agilent ELS delivers clean baselines, no drift, no solvent peaks, and much greater response than a UV detector.
It is possible to detect succinic acid down to an on-column load of 2 yg.