Agilent ELSD Tryptophan Quantification Technical Overview

This document introduces the Agilent Evaporative Light Scattering Detector (ELSD) in the analysis of non-chromophoric samples such as tryptophan and its metabolites. The ELSD can achieve more accurate detection of tryptophan and its metabolites without the need for derivatization.

Brand: Agilent
Document type: Product Leaflet
Language: English
Pages: 3
File format: PDF
File size: 525 KB
Published: 05 July, 2012
Updated: 30 September, 2023
MD5 checksum: 90DBB2D10AD137C4520B5AFB0706F7E5
PDF: Agilent ELSD Tryptophan Quantification Technical Overview PDF

About this manual

This document provides a technical overview of analyzing non-chromophoric samples, such as tryptophan and its metabolites, using High Performance Liquid Chromatography (HPLC). It highlights how the Agilent Evaporative Light Scattering Detector (ELSD) outperforms traditional detectors by not relying on the optical properties of the analyte.

What's inside

Introduction
Tryptophan Metabolism
Detection Advantages
Chromatography Conditions
Peak Identification

Frequently asked questions

What is the stated flow rate for the described HPLC analysis?

The specified flow rate for the analysis is 1 mL/min.

Which column dimensions are recommended for this method?

The recommended column has dimensions of 4.6 x 250 mm.

What eluent composition is used in the provided conditions?

The eluent consists of 40 mM Ammonium acetate at pH 6 with 7% Acetonitrile.

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