Agilent Method development on the Agilent 1290 Infinity LC using Intelligent System Emulation Technology (ISET) with subsequent transfer to an Agilent 1100 Series LC Manual

This Technical Overview shows the development of a chromatographic method for the analysis of paracetamol and its impurities using 1290 Inﬁ nity LC System with ISET. Having developed the method it was transferred to an Agilent 1100 Series Quaternary LC System.

Brand: Agilent
Document type: Other
Language: English
Pages: 8
File format: PDF
File size: 846 KB
Published: 05 July, 2012
Updated: 30 September, 2023
Models: 1100 Series, 1290 Infinity LC, 1100 Series LC
MD5 checksum: 4CDC924BC9A9B23D4EE0BA17CBC68073
PDF: Agilent Method development on the Agilent 1290 Infinity LC using Intelligent System Emulation Technology (ISET) with subsequent transfer to an Agilent 1100 Series LC Manual PDF

About this manual

Agilent's Intelligent System Emulation Technology (ISET) allows for the seamless transfer of methods between different Liquid Chromatography (LC) systems. This document demonstrates how a method developed on the high-performance Agilent 1290 Infinity LC System can be emulated to predict performance on an older Agilent 1100 Series Quaternary LC System before physical transfer.

What's inside

Introduction
Instrumentation and software
Sample
Chromatographic conditions
Method development on the 1290 Infinity LC System
Emulation of the 1100 Series Quaternary LC System using ISET
Results and discussion
Conclusion

Specifications

Column	Agilent ZORBAX SB C18, 150 x 4.6 mm, 5 µm
Mobile phase A	Water + 0.1% TFA
Mobile phase B	Acetonitrile + 0.09% TFA
Flow rate	1.0 mL/min
Detection wavelengths	220, 254, 270, 310/10 nm

Frequently asked questions

What is the retention time deviation when using ISET compared to the 1100 Series?

The retention time deviation for the 1290 Infinity LC System with ISET was between 0.5% and 1.9%.

What is the resolution deviation when using ISET compared to the 1100 Series?

The deviation of the resolution on the 1290 Infinity LC System with ISET was between 0.11% and 2.4%.

What software revision was used for the experiments?

Agilent ChemStation revision C.01.03 and ISET revision 1.0 were used.

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