This technical overview discusses the use of method translation calculators to make conversions from conventional HPLC to high-throughput HPLC and UHPLC more convenient to perform without the need for manual calculations. Both isocratic and gradient method translations are discussed, and applications are demonstrated.
With the emphasis on high-throughput liquid chromatography, this document addresses how to improve laboratory productivity by converting conventional methods to faster conditions. It explains that while standard equations exist, software packages are often used to assist with these conversions.
The text focuses specifically on HPLC method translation, covering both isocratic and gradient methods. It details the parameters involved in conversion, such as particle size, column dimensions, flow rate, and pressure, providing a practical example of translating an isocratic separation of vitamin E tocopherols to achieve faster analysis times.
The goal is to achieve faster separations with equivalent efficiency while maintaining the same elution order and resolution as the original method.
A speed increase of a factor of 5x was established using existing equipment, while also aiming to save solvent.