This document introduces Agilent Intelligent System Emulation Technology (ISET) technology, which can be used to achieve seamless method transfer from Agilent 1100/1200 Series LCs to Agilent 1290 Infi nity LC. Using ISET, the same chromatographic results can be achieved without any modifications to the instrument or changes to the original method. The document demonstrates through experiments that gradients run on an Agilent 1100 Series LC System, including typical linear gradients as well as gradients with dif- ferent slopes, steps, and isocratic parts, correlate almost 100% to gradient profi les created on the Agilent 1290 Infi nity LC System with ISET.
Agilent Intelligent System Emulation Technology (ISET) is a function within the Agilent 1290 Infinity LC System that allows users to emulate other systems for the seamless transfer of methods between LC instruments. This capability ensures that the same chromatographic results can be achieved without any modifications to the instrument or changes to the original method.
The document presents tracer experiments comparing gradient profiles from an Agilent 1100 Series Quaternary LC with those generated on an Agilent 1290 Infinity LC System with ISET. Results show that typical gradient profiles, including those with different slopes, steps, and isocratic parts, correlate almost 100% between the two systems, ensuring consistent retention times and resolution.
The system requires Agilent Chemstation revision C 01.03 and ISET revision 1.0.
Uracil at a concentration of 10 mg/L in methanol was used.
The flow rate is set to 1.5 mL/min for these gradients.