This Technical Overview demonstrates that the Agilent 1290 Infinity Quaternary Pump eliminates the need to premix the mobile phase, provides high-precision retention times and accurate quantification
This document presents a technical overview demonstrating that the Agilent 1290 Infinity Quaternary Pump eliminates the need to premix mobile phases. It compares the precision of retention times achieved with dynamically-mixed mobile phases against those obtained using premixed phases in ultrahigh performance liquid chromatography (UHPLC).
| Pump Module | Agilent 1290 Infinity Quaternary Pump (G4204A) |
|---|---|
| Autosampler Module | Agilent 1290 Infinity Autosampler with Thermostat (G4226A, G1330B) |
| Column Compartment | Agilent 1290 Infinity Thermostatted Column Compartment (G1316C) |
| Detector Module | Agilent 1260 Infinity Diode Array Detector (G4212A) |
| Flow rate | 1 mL/min |
| Column temperature | 30°C |
| Detection wavelength | 254/10 nm |
No, the pump eliminates the need to premix the mobile phase by providing highest precision using dynamically-mixed mobile phases.
Dynamically-mixed mobile phases showed better precision over several sequences and days compared to premixed phases prepared by the same or different users.
Channels A and B are used for dynamically-mixed mobile phases, while the C channel is deployed for premixed phases.