This document introduces a hybrid technology that integrates supercritical fluid chromatography and ultra-high performance liquid chromatography, which significantly improves the separation performance of complex mixtures.
The Agilent 1260 Infinity Hybrid SFC/UHPLC system is a unique solution designed to perform both supercritical fluid chromatography (SFC) and ultrahigh performance liquid chromatography (UHPLC) on a single platform. This hybrid configuration allows for orthogonal separation techniques, providing different selectivity compared to standard reversed-phase separation, which is ideal for purity analyses and impurity profiling. The system eliminates the need for two separate instruments, saving laboratory space and cost while ensuring consistent results across different modes.
| Power Range | 600-bar |
|---|---|
| SFC Backpressure Regulator Setting | 150 bar |
| UHPLC Backpressure Regulator Setting | 90 bar |
| SFC Flow Rate | 3 mL/min |
| UHPLC Flow Rate | 1.5 mL/min |
Yes, existing Agilent HPLC and SFC systems can be easily upgraded to a hybrid SFC/UHPLC system.
The system facilitates analyses in SFC or UHPLC mode up to pressures of 600 bar.