This Technical Overview discusses the compatibility of Agilent Jet Stream thermal gradient focusing technology with CE/MS. The combination of capillary electrophoresis and mass spectrometry using the Agilent triple tube coaxial sheath-flow sprayer is widely used due to its simplicity, versatility, and robustness. The study shows that with the Jet Stream source and the latest version of the CE/MS sprayer, this approach can now also be used in combination with the proprietary Agilent Jet Stream thermal gradient focusing technology. The sprayer performed well on a mass spectrometer equipped with a conventional ESI source and showed slightly improved performance compared to the standard sprayer. When the sprayer was used with an Agilent Jet Stream source, equivalent or better results were obtained compared to a conventional ESI source.
The document presents a technical comparison between standard CE/MS sprayers and an updated version compatible with Agilent Jet Stream thermal gradient focusing technology. It details the experimental setup using an Agilent 7100 CE system coupled with various mass spectrometers, including models equipped with Dual ESI sources and those with the proprietary Jet Stream source.
The text provides specific operating conditions for capillary electrophoresis (CE) and mass spectrometry (MS), covering parameters such as voltage, temperature, gas flows, and sheath liquid compositions. It reports that the updated sprayer offers improved mechanical design for better spray quality and shows equivalent or slightly increased sensitivity compared to standard configurations when used with Jet Stream sources.
| CE/MS Sprayer Part Number (Jet Stream Compatible) | G1607B |
|---|---|
| CE System Model Used | Agilent 7100 CE system |
| Capillary Length (Metamphetamine/Methadone) | 80 cm total length |
| Capillary ID (Metamphetamine/Methadone) | 50 pm id. |
| CE Voltage (Metamphetamine/Methadone) | +30 kV |
An updated triple tube coaxial sheath-flow CE/MS sprayer (p/n G1607B) is available for use with the Agilent Jet Stream source.
Yes, the Agilent Jet Stream-compatible sprayer can also be used in combination with a conventional ESI source without loss of sensitivity.
The Agilent Jet Stream-compatible sprayer showed up to two-fold higher peak areas and slightly increased performance compared to the standard sprayer.