This technical overview presents an example of a comprehensive setup for two-dimensional liquid chromatography (2D-LC) based on the Agilent 1290 Infinity 2D-LC Solution. The instrument configuration and the setup of the interface between the first and second chromatographic dimension is described in detail. The analysis of a standard sample with statistical evaluation of the results is shown to demonstrate the performance of the instrument.
This document presents a technical evaluation of the Agilent 1290 Infinity 2D-LC Solution, detailing the instrument configuration and interface setup for comprehensive two-dimensional liquid chromatography (2D-LC). It describes an example setup using two pumps, two columns, and valve switching to transfer fractions between dimensions.
The text outlines the specific equipment modules used in the evaluation, including Agilent 1290 Infinity Pumps, Autosampler, Thermostatted Column Compartment, and Diode Array Detector. It further details the experimental method, specifying solvents, flow rates, gradient profiles for both dimensions, and column types (Agilent ZORBAX RRHD) used to demonstrate separation performance.
The flow rate for the first dimension pump is set to 0.1 mL/min.
The second dimension column has dimensions of 50 x 3.0 mm with a particle size of 1.8 pm.
The injection volume is 5 pL and the sample temperature is maintained at 8 °C.