ZORBAX SB-C8 Rapid Resolution threaded columns are specially designed for higher pressure operation (up to 600 bar) and are packed with a high-performance microparticulate C8 packing for high-speed reversed-phase HPLC. The StableBond packing is made by chemically bonding a sterically protected C8 stationary phase to a specially prepared, high-purity ZORBAX porous silica microsphere. The special ZORBAX silica support is designed to reduce or eliminate strong adsorption of basic compounds. The densely covered, sterically protected, diisopropyl n-octylsilane stationary phase is chemically stable and gives longer column life. As a result, ZORBAX SB-C8 is a stable reversed-phase packing that can be used for basic, neutral, or acidic samples. It is particularly well suited for use with aggressive mobile phases (for example, pH < 2, high-ionic-strength, ion-pair additives, etc.) since the steric protection of the bonded phase resists degradation caused by such mobile phases. This material has also been shown to be stable when operated at temperatures up to 80 °C. These characteristics are particularly important for use in methods that need long-term stability and reproducibility. ZORBAX SB-C8 is especially suited in applications that use high-sensitivity detectors that require low backgrounds (for example, mass spectrome- ters).
This document is a data sheet for the Agilent ZORBAX SB-C8 Rapid Resolution threaded column, designed for high-performance microparticulate C8 packing in high-speed reversed-phase HPLC. The column utilizes StableBond packing technology, which chemically bonds a sterically protected C8 stationary phase to high-purity ZORBAX porous silica to ensure stability and longevity under aggressive mobile phase conditions.
| Maximum operating pressure | 600 bar (9,000 psi) |
|---|---|
| Maximum operating temperature | 80 °C |
| Particle pore size | 80 A |
| Compatible pH range | pH 1.8 to 8.0 |
Long-term storage should be in a pure organic solvent, preferably an aprotic liquid such as 100% acetonitrile.
Yes, the material has been shown to be stable when operated at temperatures up to 80 °C.
Check for unobstructed solvent flow up to the inlet and attempt to remove inlet debris by back-flushing 25 to 30 mL of mobile phase.