The ZORBAX Rx-Sil RRHT threaded column is specially designed for higher pressure operation (up to 600 bar) and is packed with a high-performance microparticulate silica packing for high-speed liquid-solid adsorption chromatography. ZORBAX Rx-Sil particles are produced by the agglomeration of colloidal silica to form spherical particles of uniform diameter and pore size. However, ZORBAX Rx-Sil is specially treated to create a highly homogeneous sur- face throughout the silica support. This homogeneity is achieved by utilizing ultra-high-purity silica (less than 100 ppm total of impurities such as sodium, aluminum, iron, etc.) and by applying patented technology for fully hydroxylating the silica surface. As a result, ZORBAX Rx-Sil can be used for basic, neutral, or acidic samples. It is particularly well suited for use with basic samples, since these solutes can be chromatographed without peak tailing or irreversible adsorption. The uniform, spherical, ZORBAX Rx-Sil particles have a controlled pore size of 80Å. Columns are loaded to a uni- form bed density using a proprietary, high-pressure, slurry-loading technique to give optimum column efficiency.
The document describes the Agilent ZORBAX Rx-Sil RRHT threaded column, which is packed with high-performance microparticulate silica. The particles are produced by agglutination of colloidal silica and treated to create a highly homogeneous surface using ultra-high-purity silica and patented technology for fully hydroxylating the silica surface.
| Maximum operating pressure (2.1- and 3.0-mm id) | 20,000 psi (1,300 bar) |
|---|---|
| Maximum operating pressure (4.6-mm id) | 16,000 psi (1,000 bar) |
| Maximum operating pressure (general) | 600 bar |
| Controlled pore size | 80Å |
| Minimum pH limit | pH 0.8 |
| Maximum pH limit | pH 8 |
The maximum operating temperature is typically limited only by the temperature limits of the mobile phase.
Check for obstruction up to the column inlet and attempt to remove debris by back-flushing 25-30 mL of mobile phase.
A short 3/8-inch wrench should be used to avoid damaging the end fittings.