The Agilent ZORBAX Eclipse XDB-C18 Rapid Resolution Threaded Column is a high-performance microparticulate C18 packing produced by Agilent for high-pressure operation (up to 600 bar).
This datasheet describes the Agilent ZORBAX Eclipse XDB-C18 Rapid Resolution threaded columns, which are engineered for high-pressure operation up to 600 bar. The columns utilize eXtra-Dense Bonding (XDB) technology and double endcapping to protect the ultra-pure silica support, making them suitable for separating acidic, basic, and highly polar compounds via reversed-phase HPLC.
The document provides operational guidelines, safety considerations, and storage recommendations. It specifies that the columns are compatible with water and common organic solvents but should be operated within a pH range of 3 to 8 for optimum lifetime. Detailed instructions are included for mobile phase selection, column care, and long-term storage in pure organic solvents.
| Maximum operating pressure | 600 bar (9000 psi) |
|---|---|
| Maximum operating temperature | 60 °C |
| Optimum pH range | pH 3 to 8 |
| Minimum pH limit | pH 2 |
| Maximum pH limit | pH 9 |
| Silica support surface area | 180 m²/g |
| Controlled pore size | 80 Å |
The maximum operating pressure is 600 bar (9000 psi).
Optimum lifetime and performance are obtained at a pH range of 3 to 8.
Long term storage should be in a pure organic solvent, preferably an aprotic liquid such as 100% acetonitrile.