ZORBAX Bonus-RP Rapid Resolution threaded columns are specially designed for higher pressure operation, up to 600 bar, and are packed with the unique, alkyl-amide Bonus-RP material for the separation of basic compounds. Bonus-RP uses a unique combination of densely reacted, sterically protected diisopropyl-C14 groups covalently bonded through an “embedded” amide functionality to an ultra-pure (>99.995% SiO2; Type B) ZORBAX Rx-SIL silica support. This material is then triple endcapped. This special silica support is designed to reduce or eliminate strong interaction of basic and other highly polar compounds. Bonus-RP shows different selectivity from totally alkyl or aryl stationary phases (for example, Eclipse XDB-C8, XDB-C18, and XDB-Phenyl) and may be a preferred alternative to such phases for separating basic, acidic, and other highly polar compounds by reversed-phase liquid chromatography. The embedded, highly polar amide group of the stationary phase assists in deactivating unwanted silanol interactions, while proprietary endcapping procedures complete the process to deactivate the Agilent ZORBAX Bonus-RP Rapid Resolution Threaded Column Data Sheet chromatographic surface. The more polar nature of this stationary phase usually means that a lower concentration of organic mobile phase modifier is required for compound elution compared to traditional long-chain alkyl stationary phases. This highly deactivated column packing can be used for neutral compounds and polar compounds.
This document describes the Agilent ZORBAX Bonus-RP Rapid Resolution threaded column, a specialized stationary phase for reversed-phase liquid chromatography. The column is packed with an alkyl-amide material featuring densely reacted diisopropyl-C14 groups covalently bonded to ultra-pure silica support. This unique construction reduces silanol interactions and provides superior peak shapes for basic, acidic, and highly polar compounds.
| Maximum operating pressure | 600 bar (20,000 psi for 2.1/3.0 mm id) |
|---|---|
| Operating pH range | pH 2 to 9 (optimum 2 to 8) |
| Maximum recommended temperature | 60 °C |
| Silica support surface area | 180 m²/g |
| Controlled pore size | 80 A |
It is especially suited for separating basic, acidic, and other highly polar compounds that produce poor peak shapes with traditional columns.
No, use of this column is not recommended below pH 2 or above pH 9.
Long-term storage should be in a pure organic solvent, preferably an aprotic liquid such as 100% acetonitrile.