Bio-Monolith Protein A Columns are high-performance monolithic columns that offer all the advantages of a specially designed, continuous short polymeric bed. Their inherent features enable highly reproducible separation and quantitation of immunoglobulin G (IgG) from cell culture supernatants or human plasma, at extremely high speeds. These columns are prepacked in dedicated stainless steel housings and allow user-friendly (and quick) connections to HPLC equipment. The information in this data sheet is being provided to ensure proper product care and maximal product lifetime.
This document is a datasheet for the Agilent Bio-Monolith Protein A Column, catalog number 5069-3639. It describes the column as a high-performance monolithic column designed for the rapid separation and quantitation of immunoglobulin G (IgG) from cell culture supernatants or human plasma. The column features a poly(glycidyl methacrylate-co-ethylene dimethacrylate) matrix and is prepacked in a stainless steel housing for easy connection to HPLC equipment.
The datasheet provides specific technical characteristics, including dynamic binding capacity, maximum loading capacity, and recommended working flow rates. It also outlines critical operational limits such as maximum pressure and temperature stability. Furthermore, the document details protocols for column regeneration, Cleaning in Place (CIP), and long-term storage to ensure proper product care and maximal lifetime.
| Catalog number | 5069-3639 |
|---|---|
| Column chemistry | Immunoaffinity; Protein A from Staphylococcus aureus |
| Matrix | Poly(glycidyl methacrylate-co-ethylene dimethacrylate) highly porous monolith |
| Diameter | 5.2 mm |
| Length | 4.95 mm |
| Bed volume (CV) | 0.10 mL |
| Dynamic binding capacity | > 8 mg hlgG/mL wet support |
| Maximum loading capacity | 0.4 to 0.5 mg |
| Recommended working flow rates | 0.2 to 1 mL/min |
| Maximum allowed pressure | 74 bar (7.5 MPa, 1100 psi) |
| Working temperature range | 4 to 40 °C |
| Storage temperature range | 4 to 30°C |
| Recommended pH range | 2 to 13 |
The recommended working flow rate is 0.2 to 1 mL/min (1 to 5 cm/min; 2 to 10 CV/min).
Wash with 2 mL of 0.1 M buffer containing 1 M NaCl at pH 7.0 to 8.0, followed by a low-pH solution like 10 mM HCl or 0.1 M glycine-HCl.
Wash with 0.1 M NaOH (reversing flow), then DI water, then concentrated buffer to restore pH, and finally re-equilibrate with binding mobile phase.
The maximum allowed pressure over the column is 74 bar (7.5 MPa, 1100 psi).