Bio-Monolith DEAE columns are high-performance monolith columns that offer all the advantages of a specially designed, continuous short polymeric bed. Their inherent features enable highly reproducible separations of macro bio-molecules, large proteins, DNA, and viral particles, at extremely high speeds. These columns are prepacked in dedicated stainless steel housings and allow user-friendly (and quick) connections to HPLC equipment.
The document describes the Agilent Bio-Monolith DEAE Column, catalog number 5069-3636. It is a weak anion exchanger with a poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolithic matrix designed for high-speed separation of macro bio-molecules, large proteins, DNA, and viral particles.
| Column chemistry | Weak anion exchanger; diethylamino |
|---|---|
| Matrix | Poly(glycidyl methacrylate-co-ethylene dimethacrylate) highly porous monolith |
| Ligand density | 2.3 + 0.2 mmol/g dry support |
| Matrix dimensions (Diameter) | 5.2 mm |
| Bed volume (CV) | 0.10 mL |
| Dynamic binding capacity | 25 mg BSA/mL wet support |
| Maximum loading capacity | 1.2 to 14 mg |
| Recommended working flow rates | 0.2 to 2 mL/min |
| Maximum allowed pressure | 150 bar (15 MPa, 2200 psi) |
The column should be used between 4 and 40 °C.
The recommended working pH range is 2 to 13.
Wash with at least 2 mL of a buffer containing 1M NaCl, then recondition with starting mobile phase.