Agilent ZORBAX HILIC Plus HPLC columns are non-bonded silica columns made with ZORBAX Eclipse Plus silica (high-purity, Type B silica, specially treated for good peak shape) and optimized for hydrophilic interaction chromatography (HILIC) separations. HILIC is typically used for the retention and separation of small, polar analytes. The ZORBAX HILIC Plus columns ship containing acetonitrile:water and are ready to use for HILIC separation. HILIC columns require more equilibration than reversed-phase columns and more details on this are provided in the method development section of this data sheet.
The document describes the Agilent ZORBAX HILIC Plus HPLC columns, which are non-bonded silica columns made with ZORBAX Eclipse Plus silica. These columns are specifically designed and optimized for hydrophilic interaction chromatography (HILIC) to separate small, polar analytes that may be inadequately retained on typical reversed-phase columns.
| Particle size | 3.5 um |
|---|---|
| Maximum operating pressure | 400 bar |
| Maximum operating temperature | 40 °C |
| Operating pH range | pH 1 to 8 |
They are made with ZORBAX Eclipse Plus silica, which is high-purity Type B silica specially treated for good peak shape.
Acetonitrile:water (95%:5%) is recommended as the long-term storage solvent.
Typical applications include the retention of small, polar, and/or basic analytes such as melamine, acrylamide, nucleosides, and metformin.