Bio-Monolith SO3 columns are high-performance monolithic columns that offer all the advantages of a specially designed, continuous short polymeric bed. Their inherent features enable highly reproducible separations of macro bio-molecules, antibodies, and viral particles, at extremely high speeds. These columns are prepacked in dedicated stainless steel housings and allow user-friendly (and quick) connections to HPLC equipment. The information in this data sheet is being provided to ensure proper product care and maximal product lifetime.
This document provides a technical overview of the Agilent Bio-Monolith SO3 column, a high-performance monolithic column designed for the separation of macro bio-molecules, antibodies, and viral particles. The column features a poly(glycidyl methacrylate-co-ethylene dimethacrylate) matrix with strong cation exchange chemistry.
| Matrix dimensions | Diameter: 5.2 mm; length: 4.95 mm |
|---|---|
| Bed volume (CV) | 0.10 mL |
| Ligand density | 2.3 + 0.2 mmol/g dry support |
| Maximum allowed pressure | 150 bar (15 MPa, 2200 psi) |
| Working temperature range | 4 to 40 °C |
The recommended working flow rate is between 0.2 and 2 mL/min.
Wash with at least 2 mL of a buffer containing 1M NaCl, then recondition with the starting mobile phase.
Wash with DI water and flush with a 20% ethanol solution, then seal and store at 4 to 30 °C.