Agilent Bio SEC-5 columns are designed for use with high pressure liquid chromatography (HPLC) systems. The columns have efficient and stable size exclusion capabilities for sample separation and purification. The surfaces of the columns are coated with a chemically bonded polymer layer, providing neutral and hydrophilic characteristics. The columns are available in different pore sizes and particle sizes for various applications.
This document provides a datasheet for Agilent Bio SEC-5 columns, which are designed for use with high pressure liquid chromatography (HPLC) systems. The columns are packed with 5 µm silica particles coated with a proprietary neutral, hydrophilic layer, suitable for separating various biomolecules such as antibodies, proteins, and peptides.
| Particle Size | 5 µm |
|---|---|
| Pore Sizes | 100 Å, 150 Å, 300 Å, 500 Å, 1000 Å, 2000 Å |
| pH Stability Range | 2–8.5 |
| Operating Temperature Limit | 80 °C |
| Operating Pressure Limit | 137 bar (2,000 psi) |
| Maximum Pressure | 240 bar (3,500 psi) |
| Working Flow Rate (7.8 mm id) | 0.1–1.25 mL/min |
| Working Flow Rate (4.6 mm id) | 0.1–0.4 mL/min |
The columns are suitable for antibodies, proteins, peptides, oligonucleotides, polysaccharides, polymers, biological cells, bacteria, and viral particles.
The recommended operating pressure is 137 bar (2,000 psi) for both 7.8 mm and 4.6 mm id columns.
Yes, water and organic mixtures such as acetonitrile and methanol can be used.