VariTide RPC Media is chemically and physically stable across the complete pH range. This guide is intended to help with column packing, performance and lifetime.
The document serves as a guide for personnel with knowledge of Dynamic Axial Compression (DAC) methodologies. It details the chemical and physical stability of the media across the complete pH range and provides specific instructions for preparing the packing slurry using acetonitrile:water solvent.
Key parameters for packing three sizes of laboratory Load & Lock columns are provided, including required weights of dry material and packing solvents to achieve a bed density of 0.33 g/mL. The guide also outlines the column testing procedure using UV detection at 254 nm with acetone as a test probe.
A piston packing pressure of approximately 650 psi (45 bar) is recommended.