VariPure IPE is a chemically and physically stable sorbent that can be used for ion pair removal in peptide purification applications.
The document provides guidelines for using Agilent VariPure IPE devices and loose material for ion-pair removal and freebasing applications. It is intended for personnel with good knowledge of solid phase extraction methodologies.
VariPure IPE is highly effective in removing organic acids, such as TFA and AcOH, from preparative HPLC eluent fractions and in the freebasing of isolated peptide salts. The sorbent has a nominal loading of 1.8 mmol/g of acid removal sites.
The recommended preconditioning solvent is methanol, followed by the solvent or mobile phase used in the end-user's specific application.
A slight amine odor may be detected due to a very low level of trimethylamine present in the material.
It is recommended for the purification of polar basic, hydrophobic and neutral peptide amides and acids.