Agilent LodeStars 2.7 Carboxyl Data Sheet

LodeStars 2.7 Carboxyl are superparamagnetic particles that are nominally 2.7 μm in diameter and have a surface covered with carboxylic acid groups. The carboxylic acid groups can be covalently coupled to ligands, which allows the particles to be used to identify and isolate targets in biological samples.


Agilent LodeStars 2.7 Carboxyl Data Sheet - cover page
Brand
Agilent
Category
Other
Document type
Datasheet
Language
English
Pages
4
File format
PDF
File size
661 KB
Published
04 July, 2012
Updated
01 October, 2023
MD5 checksum
A71D2B1D6397FAAE577B07D34203A811

About this manual

Agilent LodeStars 2.7 Carboxyl are superparamagnetic particles with a diameter of 2.7 um, designed for use as a solid phase in manual and automated assays. The particles feature a polymer shell with carboxylic acid groups for covalent coupling of ligands such as antibodies, antigens, and enzymes. They are supplied as a suspension in phosphate buffered saline (PBS) and are stable over a wide pH range.

What's inside

  • Chemical and Physical Properties
  • Typical Physical Characteristics
  • Principle
  • Handling and Washing
  • Coating LodeStars with Antibodies
  • Activation and Ligand Coupling
  • Antibody Coupling

Specifications

Diameter2.7 um
Concentration30 mg/mL
Bead Numbers~2.3 x 10^9/mL
Iron Content20%
Surface Area>2 m²/kg
Magnetic Mass Susceptibility>60 m³/kg
Surface ChemistryCarboxylic acid
Antibody Coupling Capacityup to 10 pg/mg

Frequently asked questions

What is the storage medium for LodeStars 2.7 Carboxyl?

They are supplied as a suspension in phosphate buffered saline (PBS) pH 7.4, 0.1% sodium azide.

How many times should LodeStars be washed prior to use?

It is recommended to wash LodeStars 2-3 times prior to use.

What is the recommended storage temperature for antibody-coated LodeStars?

They may be stable for in excess of 12 months at 2-8 °C.

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