The document introduces the features and advantages of the Agilent StrataClone cloning system, including high-efficiency yields of cloned inserts (>95%), the ability to clone both long and short amplicons, and the availability of kits for both UA and blunt-end cloning.
The document introduces the Agilent StrataClone systems as a method to clone PCR products easily using topoisomerase technology. This process eliminates the need for PCR clean-up and involves a simple 3-step procedure: adding the product to a vector mix, incubating at room temperature, and transforming into competent cells.
You must incubate the mixture for 5 minutes at room temperature.
Each product contains enough material for 20 reactions.